Fig. 4.

SJMHE1 induced IL-10 and TGF-β1 expression in CD4⁺CD25⁺ T-cells during DTH. a BALB/c mice were sensitised with OVA alone or combined with 30 μg of SJMHE1. Challenge with OVA occurred 7 days later; 24 h after the challenge, spleen and LNs from each mouse were pooled. Single-cell suspensions were prepared, and red blood cells were lysed. Intracellular expression levels of IL-10 and TGF-β1 were analysed by flow cytometry. Events were gated on CD3, CD4, and CD25 expression as indicated. Values indicate the percentage of events in the indicated quadrant. Data are expressed as the mean ± SD of 18 mice from three independent experiments; b Expression of intracellular IL-10 and TGF-β1 in gated CD4⁺CD25⁺ cells was analysed by flow cytometry. Data are representative of the experiments. Asterisks indicate significant differences analysed using the independent Student’s t-test (***P < 0.001)