Table 1.

Real-Time PCR Primer Sequences

Target Gene	Ascension No.	Forward	Reverse	Size
Collagen I	NM_000088	5′-CAG CCG CTT CAC CTA CAG C-3′	5′-TTT TGT ATT CAA TCA CTG TCT TGC C-3′	83 bp
Sox9	NM_000346	5′-CAG TAC CCG CAC TTG CAC AA-3′	5′-CTC GTT CAG AAG TCT CCA GAG CTT-3′	69 bp
Runx2	NM_004348	5′-AAC CCA CGA ATG CAC TAT CCA-3′	5′-CGG ACA TAC CGA GGG CAA TG-3′	76 bp
PPARG	NM_138711.3	5′-TGT CTC ATA ATG CCA TCA GGT TTG-3′	5′-CGC CAA CAG CTT CTC CTT CT-3′	62 bp
GAPDH	NM_002046	5′-ATG GGG AAG GTG AAG GTC G-3′	5′-TAA AAG CAG CCC TGG TGA CC-3′	70 bp

Sox9: sex-determining region Y box-9; Runx2: Runt-related transcription factor 2; PPARG: peroxisome proliferator-activated receptor gamma; GAPH: glyceraldehyde 3-phosphate dehydrogenase.