Figure 3.

Signal voids in the liver were caused by microembolization of labeled cells. Histology revealed intraportal microthrombi as morphological correlations of signal voids (A, arrow), containing Perl’s Prussian blue-positive (B), MPIO-labeled cells (C, signed with arrows). The presence of Y-chromosomes within the embolus confirmed MPIO-labeled donor cells as the origin of microthrombi (red: Y-chromosome, blue: nuclei, green: MPIO) (D). The embolus was infiltrated with macrophages (E) and labeled cells were not positive for cytokeratine (CK)-18 at day 14 after transplantation (F). Scale bars: 20 mm (A); 100 μm (B, D–F); 2.5 μm (C), 20 μm (D, inlay).