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. 2011 Oct 1;2(2):55–67. doi: 10.3727/215517911X582788

Table 1.

Primer Sequences Used for Reverse Transcriptase-PCR Analysis

Species Target Primer Sequence
Neurotrophic factors
 Human
  NGF forward 5′-ATACAGGCGGAACCACACTCAG-3′
reverse 5′-GTCCACAGTAATGTTGCGGGTC-3′
  BDNF forward 5′-AGAGGCTTGACATCATTGGCTG-3′
reverse 5′-CAAAGGCACTTGACTACTGAGCATC-3′
  GDNF forward 5′-CACCAGATAAACAAATGGCAGTGC-3′
reverse 5′-CGACAGGTCATCATCAAAGGCG-3′
  NT-3 forward 5′-GGGAGATCAAAACGGGCAAC-3′
reverse 5′-ACAAGGCACACACACAGGAC-3′
 Rat
  NGF forward 5′-ATCCACCCACCCAGTCTTCCACAT-3′
reverse 5′-GGCAGCCTGTTTGTCGTCTGTTGT-3′
  BDNF forward 5′-AGCCTCCTCTGCTCTTTCTGCTGGA-3′
reverse 5′-CTTTTGTCTATGCCCCTGCAGCCTT-3′
  GDNF forward 5′-ACTCCAATATGCCCGAAGATTATCCTG-3′
reverse 5′-CCAAACCCAAGTCAGTGACATTTAAGTG-3′
  NT-3 forward 5′-TTTCTTGCTTATCTCCGTGGCATCC-3′
reverse 5′-GGCAGGGTGCTCTGGTAATTTTCCT-3′
Pro-/anti-inflammatory cytokines
 Human
  TNF-α forward 5′-ATCTACTCCCAGGTCCTCTTCAA-3′
reverse 5′-GCAATGATCCCAAAGTAGACCT-3′
  IL-1β forward 5′-TTGACGGACCCCAAAAGATG-3′
reverse 5′-AGAAGGTGCTCATGTCCTCA-3′
  IL-6 forward 5′-GTAGCCGCCCCACACAGACAGCC-3′
reverse 5′-GCCATCTTTGGAAGGTTC-3′
  IL-10 forward 5′-ATCCAAGACAACACTACTAA-3′
reverse 5′-TAAATATCCTCAAAGTTCC-3′
 Rat
  TNF-α forward 5′-GTAGCCCACGTCGTAGCAAAC-3′
reverse 5′-TGTGGGTGAGGAGCACATAGTC-3′
  IL-1β forward 5′-CACCTTCTTTTCCTTCATCTTTG-3′
reverse 5′-GTCGTTGCTTGTCTCTCCTTGTA-3′
  IL-6 forward 5′-AAGTTTCTCTCCGCAAGAGACTTCCAG-3′
reverse 5′-AGGCAAATTTCCTGGTTATATCCAGTT-3′
  IL-10 forward 5′-CTGCTATGTTGCCTGCTCTTAC-3′
reverse 5′-TCATTCTTCACCTGCTCCACT-3′

NGF, nerve growth factor; BDNF, brain-derived neurotrophic factor; GDNF, glial cell line-derived neurotrophic factor; NT-3, neurotrophin-3; TNF, tumor necrosis factor; IL, interleukin.