Table 1.
Species | Target | Primer Sequence |
---|---|---|
Neurotrophic factors | ||
Human | ||
NGF | forward | 5′-ATACAGGCGGAACCACACTCAG-3′ |
reverse | 5′-GTCCACAGTAATGTTGCGGGTC-3′ | |
BDNF | forward | 5′-AGAGGCTTGACATCATTGGCTG-3′ |
reverse | 5′-CAAAGGCACTTGACTACTGAGCATC-3′ | |
GDNF | forward | 5′-CACCAGATAAACAAATGGCAGTGC-3′ |
reverse | 5′-CGACAGGTCATCATCAAAGGCG-3′ | |
NT-3 | forward | 5′-GGGAGATCAAAACGGGCAAC-3′ |
reverse | 5′-ACAAGGCACACACACAGGAC-3′ | |
Rat | ||
NGF | forward | 5′-ATCCACCCACCCAGTCTTCCACAT-3′ |
reverse | 5′-GGCAGCCTGTTTGTCGTCTGTTGT-3′ | |
BDNF | forward | 5′-AGCCTCCTCTGCTCTTTCTGCTGGA-3′ |
reverse | 5′-CTTTTGTCTATGCCCCTGCAGCCTT-3′ | |
GDNF | forward | 5′-ACTCCAATATGCCCGAAGATTATCCTG-3′ |
reverse | 5′-CCAAACCCAAGTCAGTGACATTTAAGTG-3′ | |
NT-3 | forward | 5′-TTTCTTGCTTATCTCCGTGGCATCC-3′ |
reverse | 5′-GGCAGGGTGCTCTGGTAATTTTCCT-3′ | |
Pro-/anti-inflammatory cytokines | ||
Human | ||
TNF-α | forward | 5′-ATCTACTCCCAGGTCCTCTTCAA-3′ |
reverse | 5′-GCAATGATCCCAAAGTAGACCT-3′ | |
IL-1β | forward | 5′-TTGACGGACCCCAAAAGATG-3′ |
reverse | 5′-AGAAGGTGCTCATGTCCTCA-3′ | |
IL-6 | forward | 5′-GTAGCCGCCCCACACAGACAGCC-3′ |
reverse | 5′-GCCATCTTTGGAAGGTTC-3′ | |
IL-10 | forward | 5′-ATCCAAGACAACACTACTAA-3′ |
reverse | 5′-TAAATATCCTCAAAGTTCC-3′ | |
Rat | ||
TNF-α | forward | 5′-GTAGCCCACGTCGTAGCAAAC-3′ |
reverse | 5′-TGTGGGTGAGGAGCACATAGTC-3′ | |
IL-1β | forward | 5′-CACCTTCTTTTCCTTCATCTTTG-3′ |
reverse | 5′-GTCGTTGCTTGTCTCTCCTTGTA-3′ | |
IL-6 | forward | 5′-AAGTTTCTCTCCGCAAGAGACTTCCAG-3′ |
reverse | 5′-AGGCAAATTTCCTGGTTATATCCAGTT-3′ | |
IL-10 | forward | 5′-CTGCTATGTTGCCTGCTCTTAC-3′ |
reverse | 5′-TCATTCTTCACCTGCTCCACT-3′ |
NGF, nerve growth factor; BDNF, brain-derived neurotrophic factor; GDNF, glial cell line-derived neurotrophic factor; NT-3, neurotrophin-3; TNF, tumor necrosis factor; IL, interleukin.