Table 2.
Proteins identified by 2D-DIGE followed by Mass Spectrometry.
| Spot number | Top ranked protein name | Accession No. | Protein MW (kDa) | Protein pI |
|---|---|---|---|---|
| 1 | Phosphoenolpyruvate carboxylase 1 | CAPP1_MAIZE | 109.22 | 5.8 |
| 2 | Putative aconitate hydratase | ACOC_ORYSJ | 98.02 | 5.7 |
| 3 | **Phosphoenolpyruvate carboxylase | gi|154146924 | 49.67 | 0 |
| 4 | Polyphosphate kinase | gi|116513638 | 83.23 | 5.8 |
| 5 | Uncharacterized protein | gi|293332375 | 90.40 | 5.7 |
| 6 | **Phosphoenolpyruvate carboxylase | gi|11991545 | 108.44 | 0 |
| 11 | **Phosphoenolpyruvate carboxykinase | gi|220938712 | 55.61 | 0 |
| 13 | PREDICTED: fibulin-2 | gi|125829574 | 92.63 | 5.4 |
| 14, 264 | *Phosphoenolpyruvate carboxylase | gi|154146806 | 49.30 | 0 |
| 15, 16, 19 | *Transketolase, chloroplastic | TKTC_MAIZE | 72.94 | 5.5 |
| 23 | Pyruvate orthophosphate dikinase | gi|380691814 | 74.79 | 0 |
| 24 | **V-type proton ATPase catalytic subunit A | VATA_MAIZE | 61.91 | 5.9 |
| 25 | 2,3-bisphosphoglycerate-independent phosphoglycerate mutase | PMGI_MAIZE | 60.58 | 5.3 |
| 31 | Succinate dehydrogenase [ubiquinone] flavoprotein subunit | DHSA_ORYSJ | 68.81 | 6.6 |
| 33, 34, 35, 36 | *Phosphoenolpyruvate carboxykinase | gi|220938712 | 55.61 | 7.6 |
| 38 | ATP synthase subunit alpha | ATPA_SACOF | 55.65 | 5.9 |
| 43 | **Phosphoenolpyruvate carboxylase | gi|154146744 | 49.43 | 0.0 |
| 44 | ATP synthase subunit alpha, mitochondrial | ATPAM_PHAVU | 55.31 | 6.5 |
| 45, 46, 48 | *ATP synthase subunit alpha, chloroplastic | ATPA_SACOF | 55.65 | 5.9 |
| 49 | ATP synthase subunit alpha, mitochondrial | ATPAM_ORYSI | 55.33 | 5.9 |
| 53, 55 | *ATP synthase subunit beta, chloroplastic | ATPB_SORBI | 53.97 | 5.3 |
| 56, 127 | *Ribulose bisphosphate carboxylase large chain | RBL_SECDI | 51.60 | 6.2 |
| 58, 59, 70 | *Ribulose bisphosphate carboxylase large chain | RBL_SETIT | 52.64 | 6.4 |
| 60, 69, 286 | *Ribulose bisphosphate carboxylase large chain | RBL_AVESA | 52.90 | 5.9 |
| 65 | #Probable sucrose-phosphate synthase 1 | SPSA1_CRAPL | 118.95 | 6.1 |
| 97, 120 | *Cysteine protease 1 precursor | gi|226496089 | 50.73 | 5.0 |
| 98 | Sedoheptulose-1,7-bisphosphatase, chloroplastic | S17P_WHEAT | 42.03 | 6.0 |
| 115 | Fructose-bisphosphate aldolase, chloroplastic | ALFC_ORYSJ | 41.98 | 6.4 |
| 128 | Unknown | gi|223974857 | 39.04 | 7.9 |
| 129 | **Chain A, Leaf Ferredoxin-Nadp+ Reductase I | gi|427930675 | 34.80 | 0 |
| 133 | Oxygen-evolving enhancer protein 1, chloroplastic | PSBO_HELAN | 34.20 | 5.4 |
| 134 | Oxygen-evolving enhancer protein 1, chloroplastic | PSBO_SOLLC | 34.92 | 5.9 |
| 140 | **Glutathione S-transferase GSTF14 | gi|46276327 | 30.71 | 0 |
| 142 | **NAD-dependent epimerase/dehydratase | gi|226499246 | 27.75 | 0 |
| 144 | **PREDICTED-proteasome subunit alpha type-7-B isoform 1 | gi|357159679 | 27.28 | 0 |
| 146 | **Predicted protein | gi|255082634 | 69.94 | 0 |
| 147 | Beta-ketoadipyl CoA thiolase | gi|171057883 | 41.78 | 7.6 |
| 208 | Hypothetical protein azo3784 | gi|119900073 | 12.28 | 8.2 |
| 209 | Ribulose bisphosphate carboxylase large chain | RBL_APHSI | 51.55 | 6.0 |
| 221, 279 | *$Putative cytochrome c oxidase subunit II PS17 | PS17_PINST | 1.70 | 9.6 |
| 225 | Oxysterol-binding protein-related protein 1D | ORP1D_ARATH | 92.27 | 6.1 |
| 226 | cytochrome b6-f complex iron-sulfur subunit | gi|195612712 | 23.97 | 8.5 |
| 230 | S-adenosylmethionine synthase | METK1_BRAJU | 43.19 | 5.5 |
| 237 | Protein CYPRO4 | CYPR4_CYNCA | 55.61 | 7.6 |
| 273, 278 | *Ras-related protein RABB1b | RAB1B_ARATH | 23.16 | 6.5 |
| 275 | 14-3-3-like protein | 1433_PEA | 29.31 | 4.7 |
| 284 | Ribulose bisphosphate carboxylase large chain | RBL_LIQST | 52.62 | 6.0 |
| 287 | **Predicted protein | gi|326529055 | 44.22 | 0 |
| 291 | Cysteine proteinase Mir3 precursor | gi|162463464 | 51.75 | 5.8 |
| 298 | **Receptor-like protein kinase | gi|2832304 | 46.55 | 0 |
| 301 | **Auxin-binding protein ABP19a-like precursor | gi|359806878 | 22.23 | 0 |
| 311 | **Hypothetical protein ZEAMMB73_006777 | gi|413933720 | 6.12 | 0 |
| 313 | **P1B-ATPase heavy-metal transporter | gi|315623028 | 115.92 | 0 |
| 314 | **Chloroplast PSI type III chlorophyll a/b-binding protein | gi|159138869 | 13.85 | 0 |
| 316 | **Hypothetical protein VITISV_010874 | gi|147843505 | 37.03 | 0 |
| 317 | **Glutathione S-transferase F8 | gi|357114170 | 25.81 | 0 |
| 321 | **Polyamine oxidase | gi|14485487 | 56.47 | 0 |
*
Different spot number corresponds to the same protein.
**
pI value 0 corresponds to the proteins with isoelectric point either below 3.0 or above 10.0 or it's mixed up with a nearby spot, thus pI value is unspecific.
#
Largest protein based on molecular weight;
$
Smallest protein based on MW.