Figure 2.

VPA enhances OLA-induced lipid accumulation in FL83B cells and induces cytotoxicity. Nile Red and Hoechst 33342 double staining showed that 24-hour VPA single treatment (a) did not significantly induce steatosis but enhanced OLA- (100 μM) induced intracellular neutral lipid accumulation (b) and lipid droplet formation (OLA = 100 μM, VPA = 1 mM) (c) in a dose-dependent manner in FL83B cells. MTT cell viability results also indicate high doses (5 and 10 mM) of VPA-induced lipotoxicity in FL83B cells (d). Values are means ± SEM of four independent experiments. ∗ above the bars refer to significant differences (P < 0.05).