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. 2016 Feb 29;2016:9576503. doi: 10.1155/2016/9576503

Figure 4.

Figure 4

VPA increases the mRNA and cell-surface protein expression levels of fatty acid translocase CD36. The mRNA expression levels of lipid transport-related genes including Cd36, Lrp, Ldlr, and Mttp were analyzed using real-time PCR (a). Relative fold changes were calculated using Ct values obtained from three independent experiments and are shown as means ± SEM. ∗ above the bars refer to significant differences (P < 0.05). The Fl83B cell-surface CD36 expression levels were analyzed by flow cytometry using mouse anti-CD36 antibody and DyLight 650-conjugated secondary antibody, and representative histograms (b) and quantitative flow cytometry data (c) are shown. ∗ above the bars refer to significant differences (P < 0.05). For background fluorescent subtraction, mouse isotype IgG antibody was used (OLA = 100 μM, VPA = 1 mM, 24 hours).