Table 6.
Percent depletion of the cysteine heptapeptide incubated with representative test chemicals in polypropylene and borosilicate glass vessels for a period of 24 h (±1) h post incubation (n = 3).
| Test chemicals | Day post incubation | Polypropylene vessel | Borosilicate glass vessel | ||
|---|---|---|---|---|---|
| Mean % depletion (±SD) | Classification of test chemical2 | Mean % depletion (±SD) | Classification of test chemical2 | ||
| DNCB (Strong sensitiser) | 0 | 88.74 (±2.5) | Moderate reactivity | 85.13 (±1.7) | Moderate reactivity |
| 1 | 81.36 (±1.0) | Moderate reactivity | 73.83 (±2.6) | Moderate reactivity | |
| 2 | 75.71 (±1.6) | Moderate reactivity | 64.09 (±2.7) | Moderate reactivity | |
| 3 | 75.83 (±0.9) | Moderate reactivity | N/A | ||
| Isoeugenol (Moderate sensitiser) | 0 | 32.84 (±7.0) | Moderate reactivity | 38.77 (±6.9) | Moderate reactivity |
| 1 | 29.41 (±3.9) | Moderate reactivity | 35.90 (±0.5) | Moderate reactivity | |
| 2 | 24.54 (±6.2) | Moderate reactivity | 30.99 (±2.5) | Moderate reactivity | |
| 3 | 28.73 (±7.0) | Moderate reactivity | N/A | ||
| Cinnamaldehyde (Moderate sensitiser) | 0 | 27.40 (±2.9) | Moderate reactivity | 35.26 (±2.3) | Moderate reactivity |
| 1 | 22.65 (±0.5) | Low reactivity* | 24.34 (±7.5) | Moderate reactivity | |
| 2 | 21.10 (±2.8) | Low reactivity* | 16.82 (±6.7) | Low reactivity* | |
| 3 | 22.66 (±4.0) | Low reactivity* | N/A | ||
| Methyl salicylate (Weak sensitiser) | 0 | 0.50 (±0.9) | No/minimal reactivity | 5.94 (±3.3) | No/minimal reactivity |
| 1 | 0.00 (±0.0) | No/minimal reactivity | 0.45 (±4.5) | No/minimal reactivity | |
| 2 | 0.95 (±1.6) | No/minimal reactivity | 1.72 (±0.9) | No/minimal reactivity | |
| 3 | 0.84 (±1.5) | No/minimal reactivity | N/A | ||
Day 0 in the table denotes the first day of sample storage in an autosampler at 4°C. The mean depletion is calculated based on the data from three replicates from each of three independent experiments.
*
change in reactivity class; N/A denotes the batch failed acceptance criteria.