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. Author manuscript; available in PMC: 2016 Oct 1.
Published in final edited form as: Hypertension. 2016 Feb 22;67(4):724–732. doi: 10.1161/HYPERTENSIONAHA.115.06925

Figure 2.

Figure 2

(A): H295R cells were transfected with pGL3-Basic or pGL3-17 Control reporter constructs and grown 24 hours post-transfection under basal or 1mM dibutyryl cAMP-stimulated conditions. The transcriptional activity of each reporter construct is displayed as a proportion of pGL3-Basic (empty vector) activity (normalised to 1). (B,C): H295R cells were transfected with reporter constructs varying in sequence from the pGL3-17 Control vector (‘CTRL’) at a single base, then grown for 24 hours post-transfection under either basal (B) or 1mM dibutyryl cAMP-stimulated (C) conditions. The transcriptional activity of each reporter construct and of a pGL3-Basic empty construct (‘E’) is displayed as a proportion of pGL3-Control activity (normalised to 1). (D): Table of reporter construct genotypes; major alleles are indicated by a superscript ‘M’. Vector alleles match the control unless otherwise indicated. All data are expressed as the mean (+/−SEM) of four independent experiments (n=4), each performed in quadruplicate.