Table 2.
Genes encoding different isozymes of 6PGDH in A. thaliana and molecular properties based on their predicted amino acid sequence. The number of amino acids corresponds to the preprocessed protein and they were used for the in silico predictions using http://web.expasy.org/protparam/ Transit peptide (TP) or targeting signal (TS) length is given in amino acids, and molecular weight (MW) of the mature.
| Properties | Locus | ||
|---|---|---|---|
| At5g41670 | At3g02360 | At1g64190 | |
| Protein accession number | BAB11473 | AEE73797 | AAF24560 |
| Number of amino acids | 487 | 486 | 487 |
| Subunit size (Da) | 53317.61 | 53577.18 | 53377.51 |
| pI | 5.62 | 7.02 | 5.34 |
| Total number of negatively charged residues (Asp + Glu) | 65 | 64 | 66 |
| Total number of positively charged residues (Arg + Lys) | 60 | 64 | 57 |
| Stability index∗ | 23.69 | 26.86 | 27.60 |
|
ε
280 (M−1 cm−1) (assuming all Cys residues are reduced) |
65320 | 63830 | 63830 |
| Aliphatic index∗∗ | 88.56 | 87.10 |
87.58 |
| Grand average of hydropathicity (GRAVY) index∗∗∗ | −0.278 | −0.283 |
−0.272 |
| Transit peptide (TP)/targeting signal (TS) | — | -SKI | — |
| Subcellular localization | Chloroplast/Cytosol | Peroxisome | Chloroplast/mitochondrion/Cytosol |
∗A protein with a stability index smaller than 40 is predicted as being stable; with a value above 40 the protein is predicted as potentially unstable.
∗∗Aliphatic index of a protein is defined as the relative volume occupied by aliphatic side chains (Ala, Val, Ile, and Leu). A positive index indicates the increase of thermostability of globular proteins.
∗∗∗GRAVY (grand average of hydropathicity) index indicates the solubility of the proteins: positive GRAVY (hydrophobic), negative GRAVY (hydrophilic).