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. 2016 Mar 4;3(2):71–84. doi: 10.18632/oncoscience.295

Figure 3. Actin binding capacity of hrRNASET2.

Figure 3

A. Indirect ELISA to assess binding capacity of hRNASET2. Serial dilutions of hrRNASET2 were incubated in actin-coated wells (ON, 4°C), which were then washed and exposed to rabbit anti-RNASET2, followed by peroxidase-conjugated goat anti-rabbit IgG. Antibody binding was then quantified using TMB-ELISA solution and measurement of optical absorbance at 650 nm. B. Quantification of the equilibrium binding constant (KD=91.5nM) of actin and hrRNASET2 by MST. Serial dilutions of hrRNASET2 were incubated with fluorescently labeled actin (RT, 5 min) before being loaded into MST glass capillaries for analysis.