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. 2016 Feb 7;8(2):245–258. doi: 10.18632/aging.100885

Figure 5. Developmental expression and ChIP assay.

Figure 5

(A) and (B) Har-CREB and -POU expression patterns during pupal stages. Brain proteins (20 μg) were extracted and used for Western blotting analysis. DP, diapause-type pupae; NP, nondiapause-type pupae. (C) Har-POU, -CREB and -c-Myc ChIP assays testing Har-HK promoter binding. In, input; IP, DNA immunoprecipitated using anti-Har-POU, -CREB and -c-Myc antibodies; Ec, empty control immunoprecipitated using pre-immune serum; Mock, mock control immunoprecipitated using anti-Har-actin antibody.