Skip to main content
. 2016 Mar 14;6:22688. doi: 10.1038/srep22688

Figure 6. Infectivity of Ad5F35 vectors on HeLa cells after their recovery at different time points from infected Jurkat cells by freeze-thawing.

Figure 6

(a) Determination of infectivity of Ad5F35 from infected Jurkat cells after freeze-thawing by flow cytometry. Jurkat cells treated with serum starvation or normal culture were infected on ice with Ad5F35-GFP at MOI of 100. Unattached virus particles were then removed and cells were transferred to 37 °C for different times and were subjected to four freeze-thaw cycles to release cell-associated virus particles. In the control settings (0 min) cells with attached viruses were lysed without incubation at 37 °C. Cell-associated virus particles were obtained and applied on HeLa cells, and the percentage of GFP-expressing cells was analyzed 48 h later by flow cytometry. (b) Percentage of GFP-expressing HeLa cells in different time point. Results represent the mean of three independent experiments with errors bars corresponding to standard error (*p < 0.05, **p < 0.01).