Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2016 Mar 14;6:22999. doi: 10.1038/srep22999

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2016, Macmillan Publishers Limited

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

PMC Copyright notice

H1299 cells were transfected with siRNA against GSK3β (a), Flag-GSK3β (b), siRNA against hnRNPK (c), or Flag-hnRNPK (d), and treated with TRAIL (20 ng/ml, 8 hours), then subjected to Western blot analysis with the indicated antibodies. (e,f) Analysis of the cell death rate by Flow cytometry. H1299 cells transfected with GSK3β siRNA or hnRNPK siRNA (e), or transfected with Flag-GSK3β or Flag-hnRNPK (f) were treated with TRAIL (20 ng/ml, 8 hours) and analyzed using Annexin V/PI double staining with a FACS Calibure flow cytometer. (g,h) Statistical analyses of the experiments shown in (e,f) respectively. The analyses were performed with the results of three independent replicates for each experiment. *p < 0.05.