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. 2016 Mar 14;6:22989. doi: 10.1038/srep22989

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(A) Left panel: Schematic of Tel5::URA3⁺ reporter strain. Right panels: Silencing assay assessing transcriptional silencing of the Tel5::URA3⁺ reporter strain in WT and sir2 Δ/Δ isolates at 30 °C, in the presence of 1 mM H₂O₂, 200 ng/μl fluconazole and at 39 °C. A URA⁺ (URA3⁺) strain was included as a control. (B) qRT-PCR analyses to measure Tel5::URA3⁺ transcript levels relative to ACT1 at 30 °C and 39 °C in WT (left panel) and sir2 Δ/Δ (right panel) strains. (C) qChIP to detect H3K9Ac levels associated with Tel5::URA3⁺ and ACT1 in WT and sir2 Δ/Δ isolats at 30 °C (Left panel) and 39 °C (Right panel). Error bars in each panel: Standard deviation (SD) of three biological replicates.