Figure 2.

(A) Effect of 3 on formalin-induced pain in rats, binned by phases. (B) Effect of 3 on aconitine-induced pain in rats summed over 60 min. (C) Effect of 3 on CFA-induced cold allodynia in mice. Terminal plasma concentrations of 3 are shown. Data are presented as mean ± SEM (n = 6–8 animals/group). Statistical analysis was performed with a two-way ANOVA, followed by a Bonferroni posthoc test (formalin assay) or by a one-way ANOVA, followed by a Dunnett’s posthoc test (aconitine and cold allodynia): *p < 0.05; **p < 0.01; ***p < 0.001 vs vehicle.