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Efficiency of autophagy ablation in chondrocytes. (A) Representative photograph of the articular cartilage of mT/mG and mT/mG-Col2Cre mice. Upon Cre recombination, the fluorescence switches from red to green. (B) Gel showing the recombination of the Atg5 gene in the articular cartilage of Atg5cKO animals. (C) Immunostaining of P62 in the joint of control and Atg5cKO mice at 2 months of age. Bars=100 µm. (D) Representative pictures of 6-month-old bones of control and Atg5cKO animals.
