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Effect of insufficient fixation on Ki-67 immunohistochemistry (IHC) in human epidermal growth factor receptor 2 (HER2)-positive cancer. Insufficient fixation caused a drastic reduction in the Ki-67 index from 33% to 5% in a HER2-positive cancer (A and B), while there was no significant change in the HER2 expression (C and D). (A and B) Ki-67 IHC; (C and D) HER2 IHC; (A and C) fixed for 48 h; (B and D) fixed for 3 h.
