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. 2016 Mar 14;6:22945. doi: 10.1038/srep22945

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(a) Immunoblot analysis of Mfa4 protein. The whole-cell lysates were separated on SDS-PAGE and probed with an Mfa4 antibody by immunoblotting. Lanes: 1, JI-1; 2, JI-1 Mfa4WT; 3, JI-1 R53A; 4, JI-1 R53K; 5, JI-1 R50A/R53A; 6, KDP112(ΔrgpA/B); 7, FMFA4 (Δmfa4, negative control)⁴⁰ (b) Immunoblot analysis of Mfa4 from purified Mfa1 fimbriae. Lanes: 1 JI-1; 2, JI-1 R53A; 3, JI-1R53K; 4, JI-1 R50A/R53A; 5, FMFA4. (C) SDS–PAGE of the purified Mfa1 fimbriae. Lanes: 1, JI-1; 2, JI-1 R53A; 3, JI-1 R53K; 4, JI-1 R50A/R53A; 5, FMFA4. Asterisks indicate the Mfa4 bands analysed by N-terminal sequencing.