Figure 7. Viral titres are decreased in the multiple packaging signal mutant virus, Δ8XSL.

48 h.p.e., virus-containing supernatant and PBS lysates from electroporated cells were titrated upon naïve cells to determine the released and intracellular viral titres, respectively, of all single pPS mutant viruses, the multiple mutant virus Δ8XSL and wildtype JFH-1 (a). To assess the rate of virion formation and release, intracellular and released virus titres were also calculated for Δ8XSL and JFH-1 at multiple time points following electroporation (b). Data represents the mean + SEM from three independent experiments.