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. 2016 Mar 14;6:23057. doi: 10.1038/srep23057

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Copyright © 2016, Macmillan Publishers Limited

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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(A) Construction of the recombinant plasmid in yeast strains. BY-T20: BY4742, ΔTrp1, Trp1::HIS3-P_PGK1-BTS1/ERG20-T_ADH1-P_TDH3-SaGGPS-T_TPI1-P_TEF1-tHMG1-T_CYC1, SGH1: BY-T20/pESC-Trp::SmCPS_ent, SGH2: BY-T20/pESC-Trp::SmKS, SGH3: BY-T20/pESC-Trp::SmCPS_ent/SmKS, SGH4: BY-T20/pESC-Trp::SmKS-SmCPS_ent. (B) Extracted ion chromatograms showing the SmCPS_ent and SmKS products. AtCPS (ent-copalol, the dephosphorylated product of ent-CPP), AtCPS + AtKS (ent-kaurene). (C) The mass spectra of the SmCPS_ent and SmKS products. (D) Relative ent-kaurene contents in the yeast strains SGH3 and SGH4 based on their peak area ratio. The data represent the mean ± SD from three independent experiments.