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. 2016 Mar 14;6:23100. doi: 10.1038/srep23100

Figure 3. miR-499-5p inhibits NSCLC metastasis in vitro and in vivo.

Figure 3

(A) Western blot analysis shows miR-499-5p regulates E-cadherin and vimentin expression. (B) Immunofluorescence analysis of E-cadherin and vimentin expression in A549 cells transfected with miR-499-5p or miR-499-5p inhibitor. (C) miR-499-5p decreases HOP62 cells invasion, while inhibition of miR-499-5p increases H522 cells invasion. (D) The miR-499-5p or miR-499-5p inhibitors stably expressed cells were injected into the tail vein of nude mice. All mice were killed after six weeks, and the lung surface nodules were counted under microscopy. Error bars represent the mean ± s.d. of three independent experiments. *p < 0.05; **p < 0.01; ***p < 0.001.