Fig. 1.

Delivery and expression of AvrPto and AvrPto variants. a A schematic of AvrPto showing the location of previously described alanine substitutions which specifically inactivate the functional domain they are in. b Hypersensitivity response in leaves of S. lycopersicum Rio Grande-PtoR (RG-PtoR) and Nicotiana tabacum L. c.v. W38 (W38). c Western blots showing protein abundance of AvrPto in D29E using a monoclonal anti-FLAG primary antibody with AvrPto proteins in part B modified to have a C-terminal FLAG epitope in the same plasmid backbone and with the same promoter. An anti-avrPto antibody used previously [31] detected non-specific proteins around 15 kDa, but gave similar results