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. Author manuscript; available in PMC: 2017 Mar 1.
Published in final edited form as: Anal Chem. 2016 Feb 18;88(5):2799–2807. doi: 10.1021/acs.analchem.5b04477

Figure 2.

Figure 2

Effect of increasing Tween-20 concentration on sample retention during buffer exchange. (A) IgG recovery from buffer exchange (n=6). Protein quantification was based on gel band intensities. For more details, see Supporting Information and Figure S-1. (B) SDS-PAGE separation of buffer-exchanged Nup84 complex obtained from affinity isolation and elution by HRV 3C protease.