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. Author manuscript; available in PMC: 2017 Mar 1.
Published in final edited form as: Anal Chem. 2016 Feb 18;88(5):2799–2807. doi: 10.1021/acs.analchem.5b04477

Figure 3.

Figure 3

Affinity isolation, peptide elution and native MS analysis of the endogenous GINS assembly from budding yeast. (A) SDS-PAGE separation and Coomassie staining to assess the post-elution sample handling steps. Elution was performed with 2 mM PEGylOx, which was later removed by buffer exchange into 150 mM ammonium acetate, 0.01 % Tween-20. (B) The native MS spectrum of the endogenous yeast GINS complex and (C) the peak series for the Ctf4 trimer. For the full spectra, see Figure S-2. (D) Spectrum showing HCD activation of the GINS complex.