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. Author manuscript; available in PMC: 2017 Mar 1.
Published in final edited form as: Anal Chem. 2016 Feb 18;88(5):2799–2807. doi: 10.1021/acs.analchem.5b04477

Figure 4.

Figure 4

Affinity isolation, protease elution and subsequent native MS analysis of the endogenous Nup84 complex from budding yeast. (A) SDS-PAGE separation and Coomassie staining to assess the post-elution sample handling steps. Elution was achieved by cleavage with the HRV 3C protease, later removed by filtration. Subsequent buffer exchange was performed with 500 mM ammonium acetate, 0.01% Tween-20. The native MS spectrum of the Nup84 complex with (B) low and (C) high in-source activation. The structural model for the Nup84 holocomplex is also shown based on integrative structural studies.34,35