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. Author manuscript; available in PMC: 2017 Mar 1.
Published in final edited form as: Anal Chem. 2016 Feb 18;88(5):2799–2807. doi: 10.1021/acs.analchem.5b04477

Figure 5.

Figure 5

Affinity isolation, protease elution and subsequent native MS analysis of the endogenous exosome assembly from budding yeast. (A) SDS-PAGE separation and Coomassie staining to assess the post-elution sample handling steps. Elution was achieved by cleavage with the TEV protease, later removed by filtration. Buffer exchange into 400 mM ammonium acetate, 0.01 % Tween-20 was then performed. (B) Representative native MS spectrum of the affinity-isolated exosome complex and the corresponding peak assignments, except for the 205-kDa subcomplex marked with *, which matched three possible subassemblies (see Table S-3).