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. Author manuscript; available in PMC: 2016 Mar 14.
Published in final edited form as: Sci Signal. 2015 Aug 18;8(390):ra81. doi: 10.1126/scisignal.aaa2819

Fig. 3. SDF2 overexpression promotes NO release by increasing the phosphorylation of eNOS.

Fig. 3

(A and C) Bar graphs illustrate the averaged measurements of NO (NO2−) released by COS7 cells expressing eNOS (A) or eNOS S1179A (C) and increasing amounts of SDF2 basally and after expression of constitutively active Akt (MyrAkt, left) or stimulation with ionomycin (right). (B) Representative immunoblot analyses and averaged densitometric data of the samples used in (A) show Akt and eNOS phosphorylation as well as SDF2 abundance. Actin was used as a loading control. Data are means ± SEM. n = 4 independent experiments. *P < 0.05 compared to untreated (Untr); #P < 0.05 compared to eNOS/eNOS S1179A, same treatment group; $P < 0.05 compared to eNOS + SDF2 1 μg.