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. Author manuscript; available in PMC: 2016 Mar 14.
Published in final edited form as: Sci Signal. 2015 Aug 18;8(390):ra81. doi: 10.1126/scisignal.aaa2819

Fig. 6. SDF2 is required for VEGF-induced recruitment of Hsp90 and CaM to the eNOS complex.

Fig. 6

(A and B) Representative immunoblot analyses (A) and averaged densitometric data (B) show the effects of SDF2 knockdown by shRNA on the VEGF-induced phosphorylation of Akt and eNOS in EA.hy926 cells. The abundance of Akt, eNOS, CaM, SDF2, and Hsp90 in whole-cell lysates (WCL) and after immunoprecipitation of eNOS (IP: eNOS) are shown. Hsp90 was used as a loading control. Control IgGs were used in control immunoprecipitation experiments (bottom left). Data are means ± SEM. n = 3 independent experiments. *P < 0.05 compared to shCTRL.