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. 2013 Jan 22;2(1):11–27. doi: 10.3390/antibiotics2010011

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© 2013 by the authors; licensee MDPI, Basel, Switzerland.

This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).

PMC Copyright notice

SA2056 interactions determined using the bacterial two-hybrid system. (a) SA2056 interactions with itself and the FemABX factors. (b) SA2056 interactions with penicillin binding proteins (PBPs). Three representative co-transformants containing the plasmids indicated were analyzed regarding β-galactosidase activity, which was determined by measuring the formation of o-nitrophenol (top). Means of three technical replicates and their standard deviation are shown. The threshold corresponding to the highest negative control value multiplied by five is indicated by a dashed line. Alternatively, the ability of co-transformants to degrade lactose to lactate was tested on MacConkey agar (bottom), where acidification of the medium leads to pink colonies.