Skip to main content
. 2015 Dec 7;4(4):627–642. doi: 10.3390/antibiotics4040627

Table 1.

Primers used in this study. The underlined sequences AGATCT and GAATTC represent the BglII and EcoRI restriction sites incorporated for cloning the wildtype gene into shuttle vector pRAB11 for complementation.

Primer Name Sequence Source or Reference
purBF 5′-GCAAGATCTATGATTGAACGCTATTCTAG-3′ This study
purBR 5′-ACGGAATTCTTATGCTAATCCAGCGCGTTCG-3′ This study
purMF 5′-GCTAGATCTATGTCTAAAGCATATGAACAATC-3′ This study
purMR 5′-ACGGAATTCTTATACCCCCAACAATTCAAT-3′ This study