Skip to main content
. Author manuscript; available in PMC: 2016 Mar 14.
Published in final edited form as: J Med Chem. 2015 Dec 10;58(24):9562–9577. doi: 10.1021/acs.jmedchem.5b01110

Figure 10. Ligand binding at wild type (dimerizing) and non-dimerizing mutant of CCK1R.

Figure 10

Shown is the ability of CE-326597 and PF-04756956 to inhibit saturable binding of 125I-BDZ-1 to wild type rat CCK1R and a non-dimerizing mutant of this receptor (rat CCK1R-Ala(317,321,325)) expressed in COS cells. The triazolobenzodiazepinones inhibited saturable binding of the BDZ radioligand in the non-dimerizing mutant construct to a greater degree than at wild type CCK1R. The X-axis values reflect the absence of ligand (0) to the left of the break, and log molar concentrations to the right of the break. Data are expressed as means ± S.E.M. of duplicate determinations from three to five independent experiments.