Figure 3.

Left: Human retinal pigmented epithelial (hRPE) cells hybridized in situ with a VEGF sense riboprobe (control), showing nonspecific cellular labeling and low background levels. Right: hRPE hybridized in situ with a VEGF antisense riboprobe, showing strong labeling of all hRPE cells and indicating VEGF expression. Reprinted with permission from Adamis AP, Shima DT, Yeo KT, et al. Synthesis and secretion of vascular permeability factor/vascular endothelial growth factor by human retinal pigment epithelial cells. Biochem Biophys Res Commun. 1993;193:631–638. Copyright 1993 Elsevier.¹⁴