Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2016 Mar 9;9:1205–1219. doi: 10.2147/OTT.S93790

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2016 Shankar et al. This work is published and licensed by Dove Medical Press Limited

The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed.

PMC Copyright notice

Protein array analysis.

Notes: Custom-designed protein array membrane was used to determine the expression of different growth factors and cytokines. The protein array membranes were imaged using luminescence imager, and each spot with signal intensity was measured using a circular ROI. The ROI size was kept consistent from array to array and spot to spot, making the variation subtle. Protein array values normalized to corresponding positive control show differential expressions of proangiogenic and growth factors based on the schedules of treatments and the added drugs. Late treatment with HET0016 caused significantly increased expression of many factors.

Abbreviations: ROI, region of interest; HET0016, N-hydroxy-N′-(4-butyl-2-methylphenyl)formamidine; G-CSF, granulocyte colony-stimulating factor; PDGF-Ra, platelet-derived growth factor receptor A; PDGF-AA, platelet-derived growth factor AA; bFGF, basic fibroblast growth factor; EGF, epidermal growth factor; EGFR, epidermal growth factor receptor; SDF-1α, stromal cell-derived factor-1 alpha; VEGFR, vascular endothelial growth factor receptor.