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. 2016 Mar 9;9:1327–1337. doi: 10.2147/OTT.S91953

Table 2.

Identification results of proteins differentially expressed between RCC and the corresponding normal tissues

Spot no Protein description Gene namea Function Accession nob Theoreticalc MW/pI Scored Coveragee Fold changef
1 Phosphoenolpyruvate carboxykinase [GTP] PCK2 Metabolism Q16822 71,438/7.57 233 22% ↓
2 78 kDa glucose-regulated protein GRP78 Molecular chaperone P11021 72,402/5.07 58 6% ↓
3 Delta-1-pyrroline-5-carboxylate dehydrogenase ALDH4A1 Metabolism P30038 62,137/8.25 224 21% ↓
4 Alpha-aminoadipic semialdehyde dehydrogenase ALDH7A1 Metabolism P49419 59,020/8.21 255 22% ↓
5 Glycine amidinotransferase GATM Metabolism P50440 48,938/8.26 167 42% ↓
6 Medium-chain specific acyl-CoA dehydrogenase ACADM Metabolism P11310 47,015/8.61 302 26% ↓
7 Argininosuccinate synthase ASS Metabolism P00966 46,786/8.08 230 33% ↓
8 Fructose-1,6-bisphosphatase 1 FBP1 Metabolism P09467 37,218/6.54 1,094 56% ↓
9 3-Hydroxyisobutyryl-CoA hydrolase HIBCH Metabolism Q6NVY1 43,797/8.38 714 44% ↓
10 Acetyl-CoA acetyltransferase, mitochondrial ACAT1 Metabolism P24752 45,456/8.98 585 39% ↓
11 Ester hydrolase C11orf54 C11orf54 Metabolism Q9H0W9 35,608/6.23 240 31% ↓
12 Glycerol-3-phosphate dehydrogenase [NAD+] GPD1 Metabolism P21695 38,171/5.81 863 60% ↓
13 Complement component 1 Q subcomponent-binding protein C1QBP Immune regulation P07021 31,749/4.74 2,109 46% ↓
14 Agmatinase, mitochondrial AGMAT Metabolism Q9BSE5 38,206/7.55 707 41% ↓
15 Calbindin CALB1 Metabolism P05937 30,291/4.7 531 46% ↓
16 Glutathione peroxidase 3 GPX3 Metabolism P22352 25,765/8.26 491 29% ↓
17 Cytochrome b–c1 complex subunit Rieske UQCRFS1 Electron transport P47985 29,934/8.55 405 37% ↓
18 ES1 protein homolog C21orf33 Metabolism P30042 28,495/8.5 201 38% ↓
19 Transgelin TAGLN Structural component P01995 22,653/8.87 158 57% ↓
20 Nucleoside diphosphate kinase B NME2 Metabolism P22392 17,401/8.52 205 40% ↓
21 Nucleoside diphosphate kinase A NME1 Metabolism P15531 17,309/5.83 246 53% ↓
22 Peptidyl-prolyl cis–trans isomerase B PPIB Metabolism P23284 23,785/9.42 511 41% ↓
23 Transthyretin TTR Hormone-binding protein P02766 15,991/5.52 122 40% ↓
24 Cytochrome c oxidase subunit 5A, mitochondrial COX5A Electron transport P20674 16,923/6.3 435 47% ↓
25 Fatty acid-binding protein, liver FABP1 Lipid transport P07148 14,256/6.6 368 53% ↓
26 10 kDa heat shock protein HSPE1 Metabolism P61604 10,925/8.89 533 52% ↓
27 Serum albumin ALB Metabolism P02768 71,317/5.92 195 16% ↑
28 Retinal dehydrogenase 1 ALDH1 Metabolism P00352 55,454/6.3 1,196 59% ↑
29 Alpha-enolase ENO1 Metabolism Q6GMP2 47,481/7.01 2,799 65% ↑
30 Glycine amidinotransferase, mitochondrial GATM Metabolism P50440 48,938/8.26 288 49% ↑
31 Pyruvate kinase isozymes M1/M2 KPYM Metabolism Q9BWB5 58,480/7.96 259 29% ↑
32 Septin-2 SEPT2 Structural component Q15019 41,689/6.15 519 40% ↑
33 Fumarylacetoacetase FAH Metabolism P16930 46,743/6.46 425 30% ↑
34 Gamma-enolase ENO2 Metabolism P09104 47,581/4.91 771 7% ↑
35 Phosphotriesterase-related protein PTER Metabolism Q96BW5 39,506/6.07 201 59% ↑
36 Alpha-soluble NSF attachment protein NAPA Electron transport P54920 33,667/5.23 228 66% ↑
37 Annexin A4 ANXA4 Calcium ion binding P09525 36,092/5.84 985 54% ↑
38 Phosphoserine aminotransferase PSAT1 Metabolism Q9Y617 40,796/7.56 249 44% ↑
39 Aldose reductase ALDR1 Metabolism P15121 36,230/6.51 293 68% ↑
40 Annexin A2 ANXA2 Calcium ion binding P07355 38,808/7.57 359 46% ↑
41 Proteasome activator complex subunit 2 PSME2 Proteolysis Q9UL46 27,555/5.54 584 66% ↑
42 Proteasome subunit alpha type-3 PSMA3 Proteolysis P25788 28,643/5.19 224 14% ↑
43 S-formylglutathione hydrolase ESD Metabolism P10768 31,956/6.54 276 47% ↑
44 Voltage-dependent anion-selective channel protein 2 VDAC2 Electron transport P45880 32,060/7.49 235 40% ↑
45 Nicotinamide N-methyltransferase NNMT Metabolism P40261 30,011/5.56 648 50% ↑
46 Glutathione S-transferase P GSTP1 Metabolism P09211 23,569/5.43 1,197 85% ↑
47 Proteasome subunit beta type-4 PSMB4 Proteolysis P28070 29,242/5.72 436 38% ↑
48 Phosphoglycerate mutase 1 PGAM1 Metabolism P18669 28,900/6.67 197 45% ↑
49 Triosephosphate isomerase TPI Metabolism P60174 26,943/6.45 1,109 55% ↑
50 Superoxide dismutase [Mn] SOD2 Electron transport P04179 24,722/8.35 442 59% ↑
51 Proteasome subunit beta type-8 PSMB8 Proteolysis P28062 30,677/7.67 273 27% ↑
52 Actin, cytoplasmic 1 ACTB Structural component P60709 42,052/5.29 194 7% ↑
53 Alpha-1-antitrypsin AAT Metabolism P01009 46,878/5.37 44 3% ↑
54 Sorcin SRI Calcium ion binding P30626 21,947/5.32 237 47% ↑
55 Ferritin heavy chain FTH1 Metabolism P02794 21,383/5.3 110 22% ↑
56 Haptoglobin HP Metabolism P00738 45,861/6.13 139 14% ↑
57 Alpha-crystallin B chain CRYAB Metabolism P02511 20,146/6.76 772 72% ↑
58 Hippocalcin-like protein 1 HPCAL1 Metabolism P37235 22,413/5.21 108 29% ↑
59 Ferritin light chain FTL Metabolism P02792 20,064/5.51 391 41% ↑
60 Eukaryotic translation initiation factor 5A-1 EIF5A Translation regulation P63241 17,053/5.08 303 42% ↑
61 Matrilysin MMP7 Metabolism P09237 29,829/7.74 181 35% ↑
62 Cofilin-1 COF1 Signal transduction P23528 18,723/8.22 364 31% ↑
63 Peptidyl-prolyl cis–trans isomerase A PPIA Protein folding P62937 18,233/7.68 548 64% ↑
64 Annexin A3 ANXA3 Calcium ion binding P12429 36,527/5.63 87 4% ↑
65 Fatty acid-binding protein, epidermal FABP5 Structural component Q01469 15,497/6.6 291 43% ↑
66 Histidine triad nucleotide-binding protein 1 HINT1 Metabolism P49773 13,905/6.43 477 65% ↑
67 Glutathione S-transferase theta-1 GSTT1 Metabolism P30711 27,489/7.01 125 13% ↑
68 Small nuclear ribonucleoprotein F SNRPF Metabolism P62306 97,76/4.7 161 40% ↑
69 SH3 domain-binding glutamic acid-rich-like protein 3 SH3BGRL3 Metabolism Q9H299 10,488/4.82 194 66% ↑
70 Protein S100-A4 S100A4 Calcium ion binding P26447 11,949/5.85 367 49% ↑
71 Protein S100-A11 S100A11 Calcium ion binding P31949 11,847/6.56 1,792 73% ↑
72 Beta-2-microglobulin B2M Immune regulation P61769 13,820/6.06 402 37% ↑
73 Ubiquitin-40S ribosomal protein S27a RPS27A Metabolism P62979 18,296/9.68 78 24% ↑

Notes:

a,b

the proteins gene name and ID from ExPASy database;

c

theoretical molecular weight (kDa) and pI from the ExPASy database;

d

probability-based MOWSE scores;

e

number of unique peptides identified by MS/MS sequencing and sequence coverage;

f

expression level in RCC compared with the corresponding normal tissues. ↑, increase; ↓, decrease.

Abbreviations: MW, molecular weight; pI, isoelectric point; MS, mass spectra; RCC, renal cell carcinoma.