FIG. 1.

Effect of cisplatin treatment on survival and clonogenic ability of agnopositive and agnonegative cells. (A) Cell viability. NIH 3T3 cells expressing JCV agnoprotein and control agnonegative NIH 3T3 cells were treated with cisplatin as described in Materials and Methods. Sets of triplicates were done for each cell line under each condition (treated and untreated). Cell viability for each cell line was evaluated by counting the cells after 4 and 5 days of treatment. (B) Clonogenic ability. Cells were treated with or without cisplatin as described for panel A. After treatment, the cells were grown for 10 to 14 days, fixed, and stained with methylene blue.