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NHEJ assay for DSB DNA repair. Agnopositive and agnonegative cells were left untreated or treated with cisplatin, and nuclear extracts were prepared as described in Materials and Methods. These were assayed by an NHEJ assay with a linear restriction-digested 3-kb plasmid. Lane *, control with no nuclear extract added. The arrow indicates the 3-kb monomeric linear plasmid substrate band. The 6- and 9-kb bands are the products of end joining and are indicated by a bracket.
