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Expression of NHEJ DNA repair proteins. Agnopositive (+) and agnonegative (−) cells were treated with cisplatin for 0, 4, 16, 24, 36, or 48 h, as indicated. Proteins were extracted and Western blotted with antibodies to Ku70, Ku80, Rad51, and Grb2 (loading control). The intensities of the Ku70 and Ku80 bands were measured by densitometry, and these data are shown in the lower panels.
