FIG. 8.

Effects of addition of full-length and C-terminal deletion mutants of agnoprotein on the end-joining activity of agnoprotein-negative cell extracts. Nuclear extracts from agnoprotein-negative NIH 3T3 cells were prepared. The extracts were incubated with GST alone or with the various GST-agnoprotein fusion proteins (full-length protein and various deletion mutants, as indicated) and assayed for NHEJ activity as described in Materials and Methods. The lane on the far left (*) is a negative control without nuclear extract. The lane on the far right (none) is a positive control that has nuclear extract but no GST-protein addition. The arrow indicates the 3-kb monomeric linear plasmid substrate band. The 6- and 9-kb bands are the products of end joining and are indicated by a bracket.