FIG. 4.
Stimulation or inhibition of infection by ConA. Virus variants CoBrB 476S, CoBrB 476S/C1.1.1 (E2-p7-pNS2-3′), C1.1.1/CoBrB, and CoBrB/C1.1.1 (Npro′-C-Erns-E1) (produced in SK6 cells) were preincubated with different concentrations of ConA for 30 min at 37°C. SK6 cells in 2-cm2 tissue culture wells were preincubated with EMEM with 300 μg of heparin per ml for 30 min at 37°C and then infected with virus. After the virus was removed and the cells were washed, the cells were incubated with 100 mM methyl-α-d-mannopyranoside for 30 min at 37°C. Subsequently, they were washed and supplied with overlay medium and stained after 24 h. Each value is the mean number of plaques calculated from two independent observations.