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. 2016 Mar 10;6:22898. doi: 10.1038/srep22898

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Copyright © 2016, Macmillan Publishers Limited

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Cell number (a) and ALP activity (b) of D1 on 300PEOT55PBT45 are shown per gradient zone at 8 and 35 days. The cell number was significantly higher in the smallest pore size compared to the other areas no matter neither the time point nor the culture conditions. At 35 days of culture the cell number in the 700 and 900/1100 zones significantly increased with respect to the previous time point. The ALP activity at 35 days under mineralization conditions showed an increase trend opposite to the pore size. Cells residing in the 900/1100 zone displayed a significantly higher ALP activity with respect to the ones located in the smallest pore zone. ^###indicates statistical significance within the same time point in graph a, p < 0.001; **, ***depict statistical significance within the same conditions p < 0.01 and p < 0.001 respectively.