Figure 4. Suppression of temperature-sensitive phenotype in mvaA mutants under high osmotic conditions.

(a,b) Overnight culture of RN4220 (wild-type), TSJY1, TSJY2, and TSJY3 was diluted 500-fold and then streaked on LB0 agar plates with or without NaCl (170, 340, or 680 mM) (a), or sucrose (290, 580, or 1160 mM) (b). The plates were incubated at 30 °C or 43 °C for 24 h.