Table 2. Kinetic parameters of S. aureus MvaA proteins.
| MvaA protein | Vmax (nmol/min/μg) |
S0.5 |
nH |
||
|---|---|---|---|---|---|
| HMG-CoA (μM) | NADPH (μM) | HMG-CoA (μM) | NADPH (μM) | ||
| Wild-type | 43 ± 1 | 11 ± 4 | 11 ± 1 | 1.4 ± 0.1 | 1.4 ± 0.1 |
| M77I | 7 ± 1c | 10 ± 1 | 29 ± 8a | 1.4 ± 0.2 | 1.1 ± 0.1a |
| A335V | 2 ± 1c | 120 ± 24b | 240 ± 120a | 1.5 ± 0.3 | 1.3 ± 0.2 |
| C366Y | 0.1 ± 0c | 26 ± 3b | 34 ± 10a | 1.2 ± 0.1a | 1.0 ± 0.1a |
By fitting with the Hill equation, the parameters were determined with S0.5 as the substrate concentration at half-maximal activity and nH as the Hill coefficient. The values are means ± S.D. of three independent experiments. ap < 0.05 (vs Wild-type). bp < 0.01 (vs Wild-type). cp < 0.000001 (vs Wild-type).