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. 2016 Feb 29;37(4):889–900. doi: 10.3892/ijmm.2016.2509

Figure 6.

Figure 6

Small interfering RNA (siRNA) gene knockdown of CHOP increases the Bcl-2 protein level and reduces apoptosis without however, impairing the anti-apoptotic effect of exendin-4 (ex-4). Mesenchymal stem cells (MSCs) were transfected with siRNA scramble/siRNA ATF-4/siRNA CHOP or treated with ex-4 prior to incubation under oxygen and glucose deprivation (OGD) conditions. The mRNA level of (A) ATF-4 and (B) CHOP was detected by RT-qPCR. (C) The apoptotic ratio was analyzed by FACS. MSCs were transfected with siRNA scramble/siRNA ATF-4 or treated with ex-4 prior to incubation under OGD conditions and the protein expression of (D) ATF-4, (E) CHOP and (F) Bcl-2 was detected by western blot analysis. MSCs were transfected with siRNA scramble/siRNA CHOP or treated with ex-4 prior to incubation under OGD conditions and the protein expression of (G) ATF-4, (H) CHOP and (I) Bcl-2 was detected by WB analysis. All experiments were repeated 3 times. *P<0.05 vs. OGD group; ^P<0.05 vs. OGD + siRNA-scramble.