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. 2004 Aug;78(16):8582–8592. doi: 10.1128/JVI.78.16.8582-8592.2004

FIG. 3.

FIG. 3.

Immunoblots of TTP and TIA-1 proteins after HSV-1 infection. (A and B) Accumulation of TTP after HSV-1 infection. (A) HeLa cells were mock infected (lane 1) or exposed to 10 PFU of HSV-1(F) per cell (lanes 2 to 4) at time zero and were collected at the indicated times after infection. Cell lysates were incubated with an antibody to TTP. (B) SK-N-SH cells were mock infected (lane 1) or exposed to 10 PFU of HSV-1(F) (lane 2) or Δα27 (lane 3), ΔUL41 (lane 4), or Δα4 (lane 5) mutant virus per cell, collected 17 h after infection, and incubated with an antibody to TTP. (C and D) Cytoplasmic accumulation of TIA-1 after HSV-1 infection. (C) Immunoblot of uninfected or HSV-1(F)-infected SK-N-SH whole-cell lysates. The cells were harvested at the indicated times after infection. (D) Immunoblot of cytoplasmic (C) and nuclear (N) fractions of uninfected or HSV-1(F)-infected HeLa, HEp-2, and SK-N-SH cell lysates incubated with a goat polyclonal antibody to TIA-1. The cells were harvested at the indicated times after infection.