Table I.
Gene | Forward | Reverse |
---|---|---|
COL-I | 5′-GAGCGGAGTACTGGATCG-3′ | 5′-GCTTCTTTTCCTTGGGGTT-3′ |
ALP | 5′-GATCATTCCCACGTTTTCACATT-3′ | 5′-TTCACCGTCCACCACCTTGT-3′ |
OCN | 5′-GAGGACCATCTTTCTGCTCACTCT-3′ | 5′-TTATTGCCCTCCTGCTTGGA-3′ |
BSP | 5′-AGGACTGCCGAAAGGAAGGTTA-3′ | 5′-AGTAGCGTGGCCGGTACTTAAA-3′ |
RUNX2 | 5′-GCACAAACATGGCCAGATTCA-3′ | 5′-AAGCCATGGTGCCCGTTAG-3′ |
β-actin | 5′-TCTGCTGGAAGGTGGACAGT-3′ | 5′-CCTCTATGCCAACACAGTGC-3′ |
COL-I, type I collagen; ALP, alkaline phosphatase; OCN, osteocalcin; BSP, bone sialoprotein; RUNX2, runt-related transcription factor 2.