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. 2016 Feb 18;37(4):901–910. doi: 10.3892/ijmm.2016.2490

Table I.

Specific primers used for RT-qPCR.

Gene Forward Reverse
COL-I 5′-GAGCGGAGTACTGGATCG-3′ 5′-GCTTCTTTTCCTTGGGGTT-3′
ALP 5′-GATCATTCCCACGTTTTCACATT-3′ 5′-TTCACCGTCCACCACCTTGT-3′
OCN 5′-GAGGACCATCTTTCTGCTCACTCT-3′ 5′-TTATTGCCCTCCTGCTTGGA-3′
BSP 5′-AGGACTGCCGAAAGGAAGGTTA-3′ 5′-AGTAGCGTGGCCGGTACTTAAA-3′
RUNX2 5′-GCACAAACATGGCCAGATTCA-3′ 5′-AAGCCATGGTGCCCGTTAG-3′
β-actin 5′-TCTGCTGGAAGGTGGACAGT-3′ 5′-CCTCTATGCCAACACAGTGC-3′

COL-I, type I collagen; ALP, alkaline phosphatase; OCN, osteocalcin; BSP, bone sialoprotein; RUNX2, runt-related transcription factor 2.