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. 2016 Feb 12;37(4):976–988. doi: 10.3892/ijmm.2016.2489

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Copyright: © Henrich et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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(A) Increased mesenchymal stem cell (MSC) concentration and (B) increased calcium deposition in femor-derived MSCs harvested using the Reamer/Irrigator/Aspirator (RIA) technique. Median and quartiles are shown. MSC concentration was determined using the colony forming unit-fibroblast (CFU-F) assay. Calcium deposition was measured by the evaluation of von Kossa staining as a percentage of black staining as described in the Materials and methods. ^*p<0.05, statistically significant difference. MSCs obtained from the femur by RIA (F-RIA), from the femur using a spoon (F-Spo), from the iliac crest using a spoon (IC-Spo) and from the iliac crest by fine-needle aspiration (IC-Asp) were analyzed.