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. 2016 Feb 12;37(4):976–988. doi: 10.3892/ijmm.2016.2489

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Copyright: © Henrich et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Expression of genes involved in BMP-2 signaling depending on the tissue site and harvest procedure of mesenchymal stem cells (MSCs) under control and osteogenic-inducing conditions (OD). Gene expression of (A) BMP2, (B) RUNX2 and (C) SMAD5 was normalized to GAPDH gene expression by the ΔCt method. Values are presented as boxplots of the median. MSCs obtained from the femur by RIA (F-RIA), from the femur using a spoon (F-Spo), from the iliac crest using a spoon (IC-Spo) and from the iliac crest by fine-needle aspiration (IC-Asp) were analyzed. ^*p<0.05. Exact p-values are additionally shown in Table I. RIA, Reamer/Irrigator/Aspirator.

Expression of genes involved in BMP-2 signaling depending on the tissue site and harvest procedure of mesenchymal stem cells (MSCs) under control and osteogenic-inducing conditions (OD). Gene expression of (A) BMP2, (B) RUNX2 and (C) SMAD5 was normalized to GAPDH gene expression by the ΔCt method. Values are presented as boxplots of the median. MSCs obtained from the femur by RIA (F-RIA), from the femur using a spoon (F-Spo), from the iliac crest using a spoon (IC-Spo) and from the iliac crest by fine-needle aspiration (IC-Asp) were analyzed. ^*p<0.05. Exact p-values are additionally shown in Table I. RIA, Reamer/Irrigator/Aspirator.