Table I.
Expression of genes involved in the osteogenic differentiation of mesenchymal stem cells (MSCs) cultured under (A) control (A) and (B) osteogenic-inducing conditions, as well as (C) intergroup comparisons according to the harvest site and harvest technique.a
| A, Control conditions | ||||
|---|---|---|---|---|
|
| ||||
| Gene | Femur (RIA) | Femur (spoon) | Iliac crest (spoon) | Iliac crest (aspirates) |
| BMP2 | p=0.05 vs. RIA | p=0.009 vs. RIA | ||
| SMAD5 | p=0.02 vs. RIA | |||
| RUNX2 | p=0.01 vs. RIA | p=0.004 vs. RIA | ||
| WNT3A | p=0.04 vs. RIA | |||
| LRP5 | p=0.03 vs. RIA | |||
| p=0.05 vs. iliac crest (spoon) | ||||
| DKK1 | p=0.04 vs. RIA | |||
| CTNNB1 | p=0.04 vs. RIA | |||
| BGLAP | p=0.05 vs. RIA | p=0.04 vs. RIA | p=0.006 vs. RIA | |
| p=0.02 vs. iliac crest (spoon) | ||||
| COL1A | p=0.02 vs. RIA | p=0.02 vs. RIA | p=0.002 vs. RIA | |
| ALPL | ||||
| B, Osteogenic-inducing conditions | ||||
|---|---|---|---|---|
|
| ||||
| Gene | Femur (RIA) | Femur (spoon) | Iliac crest (spoon) | Iliac crest (aspirates) |
| BMP2 | p=0.03 vs. RIA | p=0.004 vs. RIA | p=0.001 vs. RIA | |
| SMAD5 | p=0.05 vs. RIA | p=0.02 vs. RIA | ||
| RUNX2 | p=0.02 vs. RIA | |||
| WNT3A | ||||
| LRP5 | p=0.03 vs. RIA | |||
| DKK1 | ||||
| CTNNB1 | ||||
| BGLAP | p=0.02 vs. RIA | p=0.04 vs. RIA | p=0.002 vs. RIA | |
| COL1A | ||||
| ALPL | ||||
| C, Comparison between control conditions (cont) and osteogenic-inducing conditions (ost. diff) | ||||
|---|---|---|---|---|
|
| ||||
| Gene | Femur (RIA) cont. vs. ost. diff. | Femur (spoon) cont. vs. ost. diff. | Iliac crest (spoon) cont. vs. ost. diff. | Iliac crest (asp.) cont. vs. ost. diff. |
| BMP2 | 0.21, n.s. | 0.46, n.s. | 0.71, n.s. | 0.66, n.s. |
| SMAD5 | 0.48, n.s. | 0.81, n.s. | 0.83, n.s. | 0.46, n.s. |
| RUNX2 | 0.91, n.s. | 0.91, n.s. | 0.36, n.s. | 0.83, n.s. |
| WNT3A | 0.73, n.s. | 0.51, n.s. | 0.20, n.s. | 0.02, significant |
| LRP5 | 0.43, n.s. | 0.77, n.s. | 0.58, n.s. | 0.43, n.s. |
| DKK1 | 0.53, n.s. | 0.75, n.s. | 0.11, n.s. | 0.19, n.s. |
| CTNNB1 | 0.68, n.s. | 0.24, n.s. | 0.81, n.s. | 0.13, n.s. |
| BGLAP | 0.47, n.s. | 0.34, n.s. | 0.22, n.s. | 0.24, n.s. |
| COL1A | 0.26, n.s. | 0.91, n.s. | 0.85, n.s. | 0.12, n.s. |
| ALPL | 0.51, n.s. | 0.65, n.s. | 0.16, n.s. | 0.41, n.s. |
a
Only p-values <0.05 are presented in (A and B). The statistical evaluation of the comparison between MSCs cultivated under control and osteogenic-inducing conditions is shown in (C). Most differences found under osteogenic differentiation conditions were not significant with the exception of WNT3A gene expression in MSCs harvested from the iliac crest. n.s., no significance. RIA, Reamer/Irrigator/Aspirator; spoon, obtained using a spoon; asp., obtained by fine-needle aspiration.